The NUP98 Polyclonal Antibody (CAB0530) is a valuable tool for researchers studying the NUP98 protein, a component of the nuclear pore complex involved in nucleocytoplasmic transport and gene regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the NUP98 protein, allowing for accurate detection and analysis in a variety of cell types.NUP98, a nucleoporin protein, plays a critical role in mediating the transport of macromolecules between the nucleus and cytoplasm, as well as in regulating gene expression.
Dysregulation of NUP98 has been implicated in various diseases, including leukemia and other cancers, making it an attractive target for research in oncology and molecular biology. By studying the function and localization of NUP98, researchers can gain valuable insights into its role in cellular processes and disease pathology.
Product Name:
NUP98 Rabbit Polyclonal Antibody
SKU:
CAB0530
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 621-920 of human NUP98 (NP_624357.1).
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin.
Purification Method:
Affinity purification
Gene ID:
4928
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NUP98 antibody (CAB0530) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
