The KO-Validated ASS1 Polyclonal Antibody (CAB19878) is a powerful tool for researchers studying ASS1, a key enzyme in the urea cycle that plays a critical role in the production of arginine. This antibody, generated in rabbits, has been rigorously tested and validated for use in Western blot applications with knockout (KO) cell lines, ensuring accurate and reliable results.ASS1 is essential for maintaining nitrogen balance in the body and is often dysregulated in various diseases, including cancer. By targeting ASS1 with this antibody, researchers can study its expression levels and activity in different cell types, providing valuable insights into its role in metabolic processes and disease progression.
With its high specificity and sensitivity, the KO-Validated ASS1 Polyclonal Antibody is a valuable tool for researchers in the fields of biochemistry, oncology, and metabolism. Its versatility and reliability make it an ideal choice for studies aiming to elucidate the function of ASS1 and its potential as a therapeutic target in various disease states.
Product Name:
[KO Validated] ASS1 Rabbit Polyclonal Antibody
SKU:
CAB19878
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-412 of human ASS1 (NP_446464.1).
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
445
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and ASS1 knockout (KO) HeLa cells, using [KO Validated] ASS1 Rabbit pAb (CAB19878) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
