The [KO Validated] CD147/BSG Antibody (CAB18032) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human samples and has been validated for use in Western blot applications.BSG, also known as CD147 or EMMPRIN, is known to play a role in tumor progression, invasion, and metastasis, making it a promising target for cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] CD147/BSG Antibody
SKU:
CAB18032
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OK, 5F7, TCSF, CD147, EMPRIN, HAb18G, EMMPRIN, SG
Positive Sample:
293T
Cellular Localization:
Cell Membrane, Melanosome, Single-Pass Type I Membrane Protein.
Calculated MW:
42kDa
Observed MW:
48-58kDa
The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
682
RRID
AB_2861828
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CD147/CD147/BSG knockout (KO) 293T cells, using [KO Validated] CD147/BSG Rabbit pAb (CAB18032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from L-02 cells using CD147/BSG Rabbit pAb (CAB18032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
