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[KO Validated] BUB1 Rabbit Polyclonal Antibody (CAB18053)

SKU:
CAB18053
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€139 - €419
Frequently bought together:

Description

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Product Name:[KO Validated] BUB1 Rabbit Polyclonal Antibody
SKU:CAB18053
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1).
Sequence:MDTP ENVL QMLE AHMQ SYKG NDPL GEWE RYIQ WVEE NFPE NKEY LITL LEHL MKEF LDKK KYHN DPRF ISYC LKFA EYNS DLHQ FFEF LYNH GIGT LSSP LYIA WAGH LEAQ GELQ HASA VLQR GIQN QAEP REFL QQQY RLFQ TRLT ETHL PAQA RTSE PLHN VQVL NQMI TSKS NPGN NMAC ISKN QGSE LSGV ISSA CDKE SNME RRVI TISK SEYS VHSS LASK VDVE QVVM YCKE KLIR GESE FSFE ELRA QKYN QRRK HEQW VNED RHYM KRKE ANAF EEQL LKQK MDEL HKKL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:BUB1A; BUB1L; hBUB1; MCPH30; B1
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus, centromere, kinetochore.
Calculated MW:122kDa
Observed MW:133kDa

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:699
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit pAb (CAB18053) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.

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