The [KO Validated] CAST Antibody (CAB7634) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and reactivity with knockout (KO) validated samples, ensuring accurate and reliable results in experiments. It is designed for use in Western blot applications, enabling the detection and analysis of calpastatin protein levels in various cell types.Calpastatin plays a crucial role in controlling calpain-mediated proteolysis, a process essential for cell function and viability. Dysregulation of calpain activity has been implicated in various diseases, including neurodegenerative disorders and muscle disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] CAST Antibody
SKU:
CAB7634
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BS-17, PLACK, ST
Positive Sample:
A-549, SW480, MCF-7, HepG2, Mouse lung, Rat liver
Cellular Localization:
Cytoplasm, Cytosol, Endoplasmic Reticulum.
Calculated MW:
77kDa
Observed MW:
130kDa
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method
Affinity purification
Gene ID
831
RRID
AB_2863554
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] CAST Rabbit pAb (CAB7634) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
