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[KO Validated] PTEN Rabbit Polyclonal Antibody (CAB11193)

SKU:
CAB11193
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • KO Validated Antibodies 1 Anti-PTEN Antibody CAB11193KO Validated
  • KO Validated Antibodies 1 Anti-PTEN Antibody CAB11193KO Validated
  • KO Validated Antibodies 1 Anti-PTEN Antibody CAB11193KO Validated
  • KO Validated Antibodies 1 Anti-PTEN Antibody CAB11193KO Validated
  • KO Validated Antibodies 1 Anti-PTEN Antibody CAB11193KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PTEN Rabbit Polyclonal Antibody
SKU:CAB11193
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PTEN (NP_000305.3).
Sequence:MTAI IKEI VSRN KRRY QEDG FDLD LTYI YPNI IAMG FPAE RLEG VYRN NIDD VVRF LDSK HKNH YKIY NLCA ERHY DTAK FNCR VAQY PFED HNPP QLEL IKPF CEDL DQWL SEDD NHVA AIHC KAGK GRTG VMIC AYLL HRGK FLKA QEAL DFYG EVRT RDKK GVTI PSQR RYVY YYSY LLKN HLDY RPVA LLFH KMMF
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; PTEN
Positive Sample:HeLa,NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, PML body, Secreted.
Calculated MW:47kDa
Observed MW:54kDa

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:5728
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using PTEN Rabbit pAb (CAB11193) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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