The [KO Validated] CRKL Antibody (CAB11735) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for detecting the Crk-like protein in human samples and has been validated for use in Western blot applications.The Crk-like protein, a member of the Crk family of adaptor proteins, plays a crucial role in mediating signaling pathways that regulate cell morphology and motility. Dysregulation of Crk-like protein expression has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] CRKL Antibody
SKU:
CAB11735
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CRKL
Positive Sample:
LO2, Jurkat, SKOV3, Mouse brain, Rat heart
Cellular Localization:
Cytosol, Extrinsic Component Of Postsynaptic Membrane, Neuromuscular Junction, Nucleoplasm.
Calculated MW:
34kDa
Observed MW:
37kDa
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Purification Method
Affinity purification
Gene ID
1399
RRID
AB_2861639
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CRKL Rabbit pAb (CAB11735) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
