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[KO Validated] DNAJB1 Rabbit Polyclonal Antibody (CAB5504)

SKU:
CAB5504
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • KO Validated Antibodies 1 Anti-DNAJB1 Antibody CAB5504KO Validated
  • KO Validated Antibodies 1 Anti-DNAJB1 Antibody CAB5504KO Validated
  • KO Validated Antibodies 1 Anti-DNAJB1 Antibody CAB5504KO Validated
  • KO Validated Antibodies 1 Anti-DNAJB1 Antibody CAB5504KO Validated
  • KO Validated Antibodies 1 Anti-DNAJB1 Antibody CAB5504KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] DNAJB1 Rabbit Polyclonal Antibody
SKU:CAB5504
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
Sequence:MGKD YYQT LGLA RGAS DEEI KRAY RRQA LRYH PDKN KEPG AEEK FKEI AEAY DVLS DPRK REIF DRYG EEGL KGSG PSGG SGGG ANGT SFSY TFHG DPHA MFAE FFGG RNPF DTFF GQRN GEEG MDID DPFS GFPM GMGG FTNV NFGR SRSA QEPA RKKQ DPPV THDL RVSL EEIY SGCT KKMK ISHK RLNP DGKS IRNE DKIL TIEV KKGW KEGT KITF PKEG DQTS NNIP ADIV FVLK DKPH NIFK RDGS DVIY PARI SLRE ALCG CTVN VPTL DGRT IPVV FKDV IRPG MRRK VPGE GLPL PKTP EKRG DLII EFEV IFPE RIPQ TSRT VLEQ VLPI
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:Hdj1; Sis1; HSPF1; Hsp40; RSPH16B; B1
Positive Sample:Hep G2,C2C12,Mouse testis,Rat testis,293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, nucleolus.
Calculated MW:38kDa
Observed MW:38kDa/38KDa

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:3337
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit pAb (CAB5504) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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