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[KO Validated] EIF4G2 Rabbit Polyclonal Antibody (CAB19990)

SKU:
CAB19990
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
€139 - €419
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Description

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Product Name:[KO Validated] EIF4G2 Rabbit Polyclonal Antibody
SKU:CAB19990
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 733-850 of human EIF4G2/p97 (NP_001409.3).
Sequence:LEKE LLKQ IKLD PSPQ TIYK WIKD NISP KLHV DKGF VNIL MTSF LQYI SSEV NPPS DETD SSSA PSKE QLEQ EKQL LLSF KPVM QKFL HDHV DLQV SALY ALQV HCYN SNFP KGML LR
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:P97; AAG1; DAP5; NAT1; 97
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:adherens junction, cytosol, eukaryotic translation initiation factor 4F complex
Calculated MW:102kDa
Observed MW:97kDa

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Purification Method:Affinity purification
Gene ID:1982
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and EIF4G2/p97 knockout (KO) 293T cells, using [KO Validated] EIF4G2/p97 Rabbit pAb (CAB19990) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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