Anti-Fatty Acid Synthase Antibody (CAB0462)

Antibody Name:	Anti-Fatty Acid Synthase Antibody [KO Validated]
Antibody SKU:	CAB0462
Antibody Size:	20µL, 50µL
Application:	WB IHC IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QQQT QLNL RSLL VNPE GPTL MRLN SVQS SERP LFLV HPIE GSTT VFHS LASR LSIP TYGL QCTR AAPL DSIH SLAA YYID CIRQ VQPE GPYR VAGY SYGA CVAF EMCS QLQA QQSP APTH NSLF LFDG SPTY VLAY TQSY RAKL TPGC EAEA ETEA ICFF VQQF TDME HNRV LEAL LPLK GLEE RVAA AVDL IIKS HQGL DRQE LSFA ARSF YYKL RAAE QYTP KAKY HGNV MLLR AKTG GAYG EDLG ADYN LSQV CDGK VSVH VIEG DHRT LLEG SGLE SIIS IIHS SLAE PRVS VREG
Gene ID:	2194
Uniprot:	P49327
Cellular Location:	Cytoplasm, Melanosome
Calculated MW:	273kDa
Observed MW:	275kDa

Synonyms:

FAS, OA-519, SDR27X1, FASN

Background:

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

UniProt Protein Function:	FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
UniProt Protein Details:	Protein type:EC 1.3.1.39; EC 1.1.1.100; EC 2.3.1.39; EC 3.1.2.14; Hydrolase; EC 2.3.1.41; EC 4.2.1.59; Oxidoreductase; EC 2.3.1.38; Lyase; EC 2.3.1.85; Lipid Metabolism - fatty acid biosynthesis; Acetyltransferase Chromosomal Location of Human Ortholog: 17q25 Cellular Component: Golgi apparatus; membrane; glycogen granule; mitochondrion; cytoplasm; plasma membrane; melanosome; cytosol Molecular Function:fatty-acid synthase activity; protein homodimerization activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; drug binding; [acyl-carrier-protein] S-acetyltransferase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process; water-soluble vitamin metabolic process
NCBI Summary:	The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
UniProt Code:	P49327
NCBI GenInfo Identifier:	269849686
NCBI Gene ID:	2194
NCBI Accession:	P49327.3
UniProt Secondary Accession:	P49327,Q13479, Q16702, Q4LE83, Q6P4U5, Q6SS02, Q969R1 Q96C68, Q96IT0,
UniProt Related Accession:	P49327
Molecular Weight:	2511
NCBI Full Name:	Fatty acid synthase
NCBI Synonym Full Names:	fatty acid synthase
NCBI Official Symbol:	FASN
NCBI Official Synonym Symbols:	FAS; OA-519; SDR27X1
NCBI Protein Information:	fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1
UniProt Protein Name:	Fatty acid synthase
Protein Family:	Fatty acid synthase
UniProt Gene Name:	FASN
UniProt Entry Name:	FAS_HUMAN

	Western blot analysis of extracts of H460 cells, using Fatty Acid Synthase antibody (CAB0462) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody (CAB0462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using Fatty Acid Synthase antibody (CAB0462) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using Fatty Acid Synthase antibody (CAB0462) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using Fatty Acid Synthase antibody (CAB0462). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Fatty Acid Synthase Antibody (CAB0461)	Human Fatty Acid Synthase / FASN ELISA Kit
Anti-Fatty Acid Synthase Antibody (CAB19050)	Human FASN (Fatty Acid Synthase) CLIA Kit (HUES00269)
FASN Antibody (PACO00797)	Human FASN(Fatty Acid Synthase)ELISA Kit (HUES01592)
FASN Antibody (PACO09237)	Human Fatty acid synthase (FASN) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)