The Fatty Acid Synthase (FASN) Monoclonal Antibody (CAB19050) is a high-quality antibody developed for reliable detection and analysis of target proteins. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Fatty Acid Synthase (FASN) Monoclonal Antibody
SKU:
CAB19050
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0377
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:500 - 1:2000
IF/ICC
1:100 - 1:800
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FAS, OA-519, SDR27X1, Fatty Acid Synthase (FASN)
Positive Sample:
A549, HeLa
Cellular Localization:
Cytoplasm, Melanosome.
Calculated MW:
273kDa
Observed MW:
273kDa
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Purification Method
Affinity purification
Gene ID
2194
RRID
AB_2862543
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse skin tissue using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat fat tissue using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase (FASN) antibody (CAB19050) at a dilution of 1:500.
