The KMT2A Polyclonal Antibody (CAB1435) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been rigorously validated for knockout (KO) experiments and is highly reactive with human samples.KMT2A, also known as MLL, plays a crucial role in epigenetic regulation and is frequently implicated in leukemia and other cancers. Understanding its function and regulation is essential for unravelling the complexities of gene expression and disease development. The KO Validated KMT2A Rabbit Polyclonal Antibody allows for precise detection and analysis of KMT2A protein expression in various cell types, making it an invaluable tool for researchers in the fields of molecular biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KMT2A Polyclonal Antibody
SKU:
CAB1435
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4297
RRID
AB_2770120
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and KMT2A knockout (KO) 293T cells, using [KO Validated] KMT2A Rabbit pAb (CAB1435) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
