Anti-KMT2A Antibody (CAB1435)

Antibody Name:	Anti-KMT2A Antibody[KO Validated]
Antibody SKU:	CAB1435
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 752-949 of human KMT2A (NP_005924.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 752-949 of human KMT2A (NP_005924.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SHSM RTRS GRLS SSEL SPLT PPSS VSSS LSIS VSPL ATSA LNPT FTFP SHSL TQSG ESAE KNQR PRKQ TSAP AEPF SSSS PTPL FPWF TPGS QTER GRNK DKAP EELS KDRD ADKS VEKD KSRE RDRE REKE NKRE SRKE KRKK GSEI QSSS ALYP VGRV SKEK VVGE DVAT SSSA KKAT GRKK SSSH DSGT DITS VT
Gene ID:	4297
Uniprot:	Q03164
Cellular Location:	Nucleus
Calculated MW:	427kDa/431kDa/432kDa
Observed MW:	200KDa

	Western blot analysis of extracts of various cell lines, using KMT2A Rabbit pAb (CAB1435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts of C6 cells, using KMT2A Rabbit pAb (CAB1435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Western blot analysis of extracts of 293T cells, using KMT2A antibody (CAB1435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of HeLa cells using KMT2A antibody (CAB1435) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using KMT2A antibody (CAB1435) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Synonyms:

KMT2A, ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1, MLL1A, TRX1, WDSTS, MLL-AF9, MLL/GAS7, TET1-MLL

Background:

This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	MLL: Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis. Chromosomal aberrations involving MLL are a cause of acute leukemias. Translocation t(1;11)(q21;q23) with MLLT11/AF1Q; translocation t(3;11)(p21;q23) with NCKIPSD/AF3p21; translocation t(3,11)(q25,q23) with GMPS; translocation t(4;11)(q21;q23) with AFF1/MLLT2/AF4; insertion ins(5;11)(q31;q13q23) with AFF4/AF5Q31; translocation t(5;11)(q12;q23) with AF5-alpha/CENPK; translocation t(6;11)(q27;q23) with MLLT4/AF6; translocation t(9;11)(p22;q23) with MLLT3/AF9; translocation t(10;11)(p11.2;q23) with ABI1; translocation t(10;11)(p12;q23) with MLLT10/AF10; t(11;15)(q23;q14) with CASC5 and ZFYVE19; translocation t(11;17)(q23;q21) with MLLT6/AF17; translocation t(11;19)(q23;p13.3) with ELL; translocation t(11;19)(q23;p13.3) with MLLT1/ENL; translocation t(11;19)(q23;p23) with GAS7; translocation t(X;11)(q13;q23) with FOXO4/AFX1. Translocation t(3;11)(q28;q23) with LPP. Translocation t(10;11)(q22;q23) with TET1. Translocation t(9;11)(q34;q23) with DAB2IP. Translocation t(4;11)(p12;q23) with FRYL. Fusion proteins MLL-MLLT1, MLL-MLLT3 and MLL-ELL interact with PPP1R15A and, on the contrary to unfused MLL, inhibit PPP1R15A-induced apoptosis. A chromosomal aberration involving MLL may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with SEPT11. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase, protein lysine; Transcription factor; Oncoprotein; Cell cycle regulation; EC 2.1.1.43; Methyltransferase Chromosomal Location of Human Ortholog: 11q23 Cellular Component: nucleoplasm; histone methyltransferase complex; cytoplasm; nucleus Molecular Function:identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; AT DNA binding; unmethylated CpG binding; histone lysine N-methyltransferase activity (H3-K4 specific); chromatin binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; embryonic hemopoiesis; apoptosis; positive regulation of transcription, DNA-dependent; histone H3-K4 methylation; positive regulation of transporter activity; positive regulation of histone H3-K4 methylation; anterior/posterior pattern formation; negative regulation of cell proliferation; DNA methylation; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression Disease: Hairy Elbows, Short Stature, Facial Dysmorphism, And Developmental Delay
NCBI Summary:	This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010]
UniProt Code:	Q03164
NCBI GenInfo Identifier:	146345435
NCBI Gene ID:	4297
NCBI Accession:	Q03164.5
UniProt Related Accession:	Q03164
Molecular Weight:
NCBI Full Name:	Histone-lysine N-methyltransferase 2A
NCBI Synonym Full Names:	lysine methyltransferase 2A
NCBI Official Symbol:	KMT2A
NCBI Official Synonym Symbols:	HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS
NCBI Protein Information:	histone-lysine N-methyltransferase 2A
UniProt Protein Name:	Histone-lysine N-methyltransferase 2A
UniProt Synonym Protein Names:	ALL-1; CXXC-type zinc finger protein 7; Myeloid/lymphoid or mixed-lineage leukemia; Myeloid/lymphoid or mixed-lineage leukemia protein 1; Trithorax-like protein; Zinc finger protein HRXCleaved into the following 2 chains:MLL cleavage product N320Alternative name(s):N-terminal cleavage product of 320 kDa; p320
UniProt Gene Name:	KMT2A
UniProt Entry Name:	KMT2A_HUMAN