The [KO Validated] MyD88 Monoclonal Antibody (CAB19082) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through rabbit monoclonal technology, offers high specificity and sensitivity in detecting MyD88 in various cell types and tissues.MyD88 is a critical adaptor protein involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling, leading to the activation of NF-κB and MAPK pathways in response to pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs).
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] MyD88 Monoclonal Antibody
SKU:
CAB19082
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC52507
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
WM1, IMD68, MYD88D, 88
Positive Sample:
HeLa, A549
Cellular Localization:
Cytoplasm.
Calculated MW:
15kDa/20kDa/28kDa/31kDa/33kDa/34kDa
Observed MW:
33kDa
This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4615
RRID
AB_2862574
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and MyD88 knockout (KO) HeLa cells, using [KO Validated] MyD88 Rabbit mAb (CAB19082) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of lysates from A549 cells, using Aquaporin-4 (AQP4) Rabbit mAb (CAB19082) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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