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Anti-SFN Antibody (CAB1026)

SKU:
CAB1026
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-SFN Antibody CAB1026
  • Cell Biology Antibodies 1 Anti-SFN Antibody CAB1026
  • Cell Biology Antibodies 1 Anti-SFN Antibody CAB1026
  • Cell Biology Antibodies 1 Anti-SFN Antibody CAB1026
  • Cell Biology Antibodies 1 Anti-SFN Antibody CAB1026
€139 - €419
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Description

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Antibody Name:Anti-SFN Antibody
Antibody SKU:CAB1026
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, MCF7, A-431, Mouse lung, Mouse kidney, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MERA SLIQ KAKL AEQA ERYE DMAA FMKG AVEK GEEL SCEE RNLL SVAY KNVV GGQR AAWR VLSS IEQK SNEE GSEE KGPE VREY REKV ETEL QGVC DTVL GLLD SHLI KEAG DAES RVFY LKMK GDYY RYLA EVAT GDDK KRII DSAR SAYQ EAMD ISKK EMPP TNPI RLGL ALNF SVFH YEIA NSPE EAIS LAKT TFDE AMAD LHTL SEDS YKDS TLIM QLLR DNLT LWTA DNAG EEGG EAPQ EPQS
Gene ID:2810
Uniprot:P31947
Cellular Location:Cytoplasm, Nucleus, Secreted
Calculated MW:24kDa/27kDa
Observed MW:28KDa
Synonyms:SFN, YWHAS, stratifin
Background:
UniProt Protein Function:14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; cytosol; nucleus

Molecular Function:protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; regulation of epidermal cell division; release of cytochrome c from mitochondria; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth

UniProt Code:P31947
NCBI GenInfo Identifier:398953
NCBI Gene ID:2810
NCBI Accession:P31947.1
UniProt Secondary Accession:P31947,Q6FH30, Q6FH51, Q96DH0,
UniProt Related Accession:P31947
Molecular Weight:24,336 Da
NCBI Full Name:14-3-3 protein sigma
NCBI Synonym Full Names:stratifin
NCBI Official Symbol:SFN  
NCBI Official Synonym Symbols:YWHAS  
NCBI Protein Information:14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1
UniProt Protein Name:14-3-3 protein sigma
UniProt Synonym Protein Names:Epithelial cell marker protein 1; Stratifin
Protein Family:14-3-3 protein
UniProt Gene Name:SFN  
UniProt Entry Name:1433S_HUMAN
Anti-SFN Antibody (CAB1026)Western blot analysis of extracts of various cell lines, using SFN antibody (CAB1026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-SFN Antibody (CAB1026)Western blot analysis of extracts of various cell lines, using SFN antibody (CAB1026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-SFN Antibody (CAB1026)Immunohistochemistry of paraffin-embedded rat skin using SFN Rabbit pAb (CAB1026) at dilution of 1:100 (40x lens).
Anti-SFN Antibody (CAB1026)Immunohistochemistry of paraffin-embedded human skin using SFN Rabbit pAb (CAB1026) at dilution of 1:100 (40x lens).
Anti-SFN Antibody (CAB1026)Immunohistochemistry of paraffin-embedded mouse skin using SFN Rabbit pAb (CAB1026) at dilution of 1:100 (40x lens).

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