Anti-STAT3 Antibody (CAB19566)

Antibody Name:	Anti-STAT3 Antibody [KO Validated]
Antibody SKU:	CAB19566
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human STAT3.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, 293T, Mouse thymus, Mouse kidney, Rat brain, HeLa

Immunogen:	A synthesized peptide derived from human STAT3.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6774
Uniprot:	P40763
Cellular Location:
Calculated MW:	88kDa
Observed MW:	82KDa/88KDa

	Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (CAB19566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
	Western blot analysis of extracts of various cell lines, using STAT3 antibody (CAB19566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
	Western blot analysis of extracts of various cell lines, using STAT3 antibody (CAB19566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Synonyms:

ADMIO, ADMIO1, APRF, HIES, STAT3, Stat3

Background:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

UniProt Protein Function:	STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: nucleoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; nucleus; cytosol Molecular Function:protein dimerization activity; ligand-dependent nuclear receptor activity; protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; protein phosphatase binding; transcription factor binding; protein kinase binding; transcription factor activity; glucocorticoid receptor binding; CCR5 chemokine receptor binding Biological Process: transcription from RNA polymerase II promoter; viral reproduction; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; response to drug; nervous system development; intracellular receptor-mediated signaling pathway; eating behavior; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; positive regulation of transcription from RNA polymerase II promoter; cell motility; eye photoreceptor cell differentiation; phosphorylation Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant; Autoimmune Disease, Multisystem, Infantile-onset
NCBI Summary:	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P40763
NCBI GenInfo Identifier:	48429227
NCBI Gene ID:	6774
NCBI Accession:	P40763.2
UniProt Secondary Accession:	P40763,O14916, Q9BW54, A8K7B8, K7ENL3,
UniProt Related Accession:	P40763
Molecular Weight:	770
NCBI Full Name:	Signal transducer and activator of transcription 3
NCBI Synonym Full Names:	signal transducer and activator of transcription 3 (acute-phase response factor)
NCBI Official Symbol:	STAT3
NCBI Official Synonym Symbols:	APRF; HIES; ADMIO
NCBI Protein Information:	signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
UniProt Protein Name:	Signal transducer and activator of transcription 3
UniProt Synonym Protein Names:	Acute-phase response factor
Protein Family:	Signal transducer and activator of transcription
UniProt Gene Name:	STAT3
UniProt Entry Name:	STAT3_HUMAN

Antibodies	ELISA Kits
Anti-STAT3 Antibody (CAB11216)	Human STAT3 ELISA Kit
Anti-STAT3 Antibody (CAB1192)[KO Validated]	Human STAT3 (Signal Transducer and Activator of Transcription 3) CLIA Kit (HUES01131)
Anti-STAT3 Antibody (CAB15729)	Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit
Anti-STAT3 Antibody (CAB5511)	STAT3 Transcription Factor Activity Assay
Anti-Phospho-Stat3-Y705 Antibody (CABP0070)	STAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)