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Anti-TSC22D3 Antibody (CAB19911)[KO Validated]

SKU:
CAB19911
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-TSC22D3 Antibody [KO Validated]
Antibody SKU:CAB19911
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human TSC22D3.
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human TSC22D3.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1831
Uniprot:Q99576
Cellular Location:Cytoplasm, Nucleus
Calculated MW:13kDa/14kDa/22kDa
Observed MW:16KDa
Anti-TSC22D3 Antibody [KO Validated] (CAB19911)Western blot analysis of extracts from normal (control) and TSC22D3 knockout (KO) 293T cells, using TSC22D3 antibody (CAB19911) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:TSC22D3, DIP, DSIPI, GILZ, TSC-22R
Background:This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor ?B. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:GILZ: Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities. Belongs to the TSC-22/Dip/Bun family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: cytosol

Biological Process: regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Code:Q99576
NCBI GenInfo Identifier:14195584
NCBI Gene ID:1831
NCBI Accession:Q99576.2
UniProt Secondary Accession:Q99576,Q5H9S3, Q5JRI9, Q6FIH6, Q8NAI1, Q8WVB9, Q9UBN5 Q9UG13,
UniProt Related Accession:Q99576
Molecular Weight:13,286 Da
NCBI Full Name:TSC22 domain family protein 3
NCBI Synonym Full Names:TSC22 domain family member 3
NCBI Official Symbol:TSC22D3  
NCBI Official Synonym Symbols:DIP; GILZ; DSIPI; TSC-22R  
NCBI Protein Information:TSC22 domain family protein 3
UniProt Protein Name:TSC22 domain family protein 3
UniProt Synonym Protein Names:DSIP-immunoreactive peptide; Protein DIP; hDIP; Delta sleep-inducing peptide immunoreactor; Glucocorticoid-induced leucine zipper protein; GILZ; TSC-22-like protein; TSC-22-related protein; TSC-22R
Protein Family:TSC22 domain family protein
UniProt Gene Name:TSC22D3  
UniProt Entry Name:T22D3_HUMAN

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