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[KO Validated] 14-3-3 gamma Rabbit Polyclonal Antibody (CAB18060)

SKU:
CAB18060
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] 14-3-3 gamma Rabbit Polyclonal Antibody
SKU:CAB18060
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human 14-3-3 gamma (P61981).
Sequence:VLSL LDNY LIKN CSET QYES KVFY LKMK GDYY RYLA EVAT GEKR ATVV ESSE KAYS EAHE ISKE HMQP THPI RLGL ALNY SVFY YEIQ NAPE QACH LAKT A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:DEE56; EIEE56; PPP1R170; 14-3-3GAMMA; ma
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:28kDa
Observed MW:28kDa

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Purification Method:Affinity purification
Gene ID:7532
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and 14-3-3 gamma knockout (KO) 293T cells, using [KO Validated] 14-3-3 gamma Rabbit pAb (CAB18060) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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