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Anti-LMX1B Antibody (CAB6386)

SKU:
CAB6386
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-LMX1B Antibody CAB6386
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-LMX1B Antibody CAB6386
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Description

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Antibody Name:Anti-LMX1B Antibody
Antibody SKU:CAB6386
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 136-395 of human LMX1B (NP_002307.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 136-395 of human LMX1B (NP_002307.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YHLG CFCC CVCE RQLR KGDE FVLK EGQL LCKG DYEK EKDL LSSV SPDE SDSV KSED EDGD MKPA KGQG SQSK GSGD DGKD PRRP KRPR TILT TQQR RAFK ASFE VSSK PCRK VRET LAAE TGLS VRVV QVWF QNQR AKMK KLAR RHQQ QQEQ QNSQ RLGQ EVLS SRME GMMA SYTP LAPP QQQI VAME QSPY GSSD PFQQ GLTP PQMP GNDS IFHD IDSD TSLT SLSD CFLG SSDV GSLQ ARVG NPID RLYS MQSS YFAS
Gene ID:4010
Uniprot:O60663
Cellular Location:Nucleus
Calculated MW:44kDa/45kDa
Observed MW:45kDa
Synonyms:LMX1B, LMX1.2, NPS1
Background:This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:LMX1B: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. Defects in LMX1B are the cause of nail-patella syndrome (NPS); also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleus

Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding; transcription factor activity

Biological Process: collagen fibril organization; transcription, DNA-dependent; in utero embryonic development; multicellular organismal development; neuron migration; cerebellum morphogenesis; limb morphogenesis; neuron differentiation; cell proliferation; organ growth; regulation of transcription, DNA-dependent; dorsal/ventral pattern formation; midbrain development; positive regulation of transcription from RNA polymerase II promoter; central nervous system neuron development

Disease: Nail-patella Syndrome

NCBI Summary:This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Code:O60663
NCBI GenInfo Identifier:485956425
NCBI Gene ID:4010
NCBI Accession:O60663.3
UniProt Secondary Accession:O60663,O75463, Q5JU95, Q6ISC9, F8W7W6,
UniProt Related Accession:O60663
Molecular Weight:402
NCBI Full Name:LIM homeobox transcription factor 1-beta
NCBI Synonym Full Names:LIM homeobox transcription factor 1, beta
NCBI Official Symbol:LMX1B  
NCBI Official Synonym Symbols:NPS1; LMX1.2  
NCBI Protein Information:LIM homeobox transcription factor 1-beta; LMX-1.2; LIM/homeobox protein 1.2; LIM/homeobox protein LMX1B
UniProt Protein Name:LIM homeobox transcription factor 1-beta
UniProt Synonym Protein Names:LIM/homeobox protein 1.2; LMX-1.2; LIM/homeobox protein LMX1B
Protein Family:LIM/homeobox protein
UniProt Gene Name:LMX1B  
UniProt Entry Name:LMX1B_HUMAN
Anti-LMX1B Antibody (CAB6386)Western blot analysis of extracts of BT-474 cells, using LMX1B antibody (CAB6386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-LMX1B Antibody (CAB6386)Immunohistochemistry of paraffin-embedded human liver cancer using LMX1B antibody (CAB6386) at dilution of 1:200 (40x lens).

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