Anti-LSM14A Antibody (CAB16682)

Antibody Name:	Anti-LSM14A Antibody
Antibody SKU:	CAB16682
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human LSM14A

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat liver

Immunogen:	A synthetic peptide of human LSM14A
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	26065
Uniprot:	Q8ND56
Cellular Location:	Cytoplasm, P-body
Calculated MW:	46kDa/50kDa
Observed MW:	50kDa

Synonyms:

LSM14A, C19orf13, FAM61A, RAP55, RAP55A

Background:

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Mar 2008]

UniProt Protein Function:	alphaSNBP(A): Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs. Belongs to the LSM14 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation; RNA-binding Chromosomal Location of Human Ortholog: 19q13.11 Cellular Component: intracellular membrane-bound organelle; stress granule; cytoplasm Molecular Function:single-stranded RNA binding; double-stranded DNA binding; double-stranded RNA binding Biological Process: regulation of translation; multicellular organismal development; cytoplasmic mRNA processing body assembly
NCBI Summary:	Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Mar 2008]
UniProt Code:	Q8ND56
NCBI GenInfo Identifier:	71648673
NCBI Gene ID:	26065
NCBI Accession:	Q8ND56.3
UniProt Related Accession:	Q8ND56
Molecular Weight:
NCBI Full Name:	Protein LSM14 homolog A
NCBI Synonym Full Names:	LSM14A mRNA processing body assembly factor
NCBI Official Symbol:	LSM14A
NCBI Official Synonym Symbols:	RAP55; FAM61A; RAP55A; C19orf13
NCBI Protein Information:	protein LSM14 homolog A
UniProt Protein Name:	Protein LSM14 homolog A
UniProt Synonym Protein Names:	Protein FAM61A; Protein SCD6 homolog; Putative alpha-synuclein-binding protein; AlphaSNBP; RNA-associated protein 55A; hRAP55; hRAP55A
UniProt Gene Name:	LSM14A
UniProt Entry Name:	LS14A_HUMAN

	Western blot analysis of extracts of various cell lines, using LSM14A Rabbit pAb (CAB16682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using LSM14A antibody (CAB16682) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

LSM14A Antibody (PACO10300)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)