Anti-LYZ Antibody (CAB2503)

Antibody Name:	Anti-LYZ Antibody
Antibody SKU:	CAB2503
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-148 of human LYZ (NP_000230.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	SW480, HL-60, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-148 of human LYZ (NP_000230.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVFE RCEL ARTL KRLG MDGY RGIS LANW MCLA KWES GYNT RATN YNAG DRST DYGI FQIN SRYW CNDG KTPG AVNA CHLS CSAL LQDN IADA VACA KRVV RDPQ GIRA WVAW RNRC QNRD VRQY VQGC GV
Gene ID:	4069
Uniprot:	P61626
Cellular Location:	Secreted
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

LYZ, LYZF1, LZM, lysozyme

Background:

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.

UniProt Protein Function:	LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family.
UniProt Protein Details:	Protein type:EC 3.2.1.17; Endoplasmic reticulum; Secreted, signal peptide; Vesicle; Secreted; Hydrolase Chromosomal Location of Human Ortholog: 12q15 Cellular Component: extracellular space; extracellular region Molecular Function:identical protein binding; lysozyme activity Biological Process: retinal homeostasis; metabolic process; cytolysis; defense response to bacterium; inflammatory response Disease: Amyloidosis, Familial Visceral
NCBI Summary:	This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]
UniProt Code:	P61626
NCBI GenInfo Identifier:	48428995
NCBI Gene ID:	4069
NCBI Accession:	P61626.1
UniProt Related Accession:	P61626
Molecular Weight:	~ 17kDa
NCBI Full Name:	Lysozyme C
NCBI Synonym Full Names:	lysozyme
NCBI Official Symbol:	LYZ
NCBI Official Synonym Symbols:	LZM; LYZF1
NCBI Protein Information:	lysozyme C
UniProt Protein Name:	Lysozyme C
UniProt Synonym Protein Names:	1,4-beta-N-acetylmuramidase C
UniProt Gene Name:	LYZ
UniProt Entry Name:	LYSC_HUMAN

	Western blot analysis of extracts of various cell lines, using LYZ Antibody (CAB2503) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using LYZ antibody (CAB2503) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using LYZ antibody (CAB2503) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using LYZ antibody (CAB2503) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-LYZ Antibody (CAB10972)	Human Lysozyme ELISA Kit
Anti-LYZ Antibody (CAB12919)	Human LZM (Lysozyme) CLIA Kit (HUES01034)
Anti-LYZ Antibody (CAB13511)	Human Lysozyme C (LYZ) ELISA Kit
Anti-Lysozyme Antibody (CAB0641)
LYZ Antibody (PACO10318)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)