Anti-MAGOH Antibody (CAB6035)

Antibody Name:	Anti-MAGOH Antibody
Antibody SKU:	CAB6035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human MAGOH (NP_002361.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, HeLa, MCF7, Jurkat, Mouse spleen, Mouse thymus, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human MAGOH (NP_002361.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESD FYLR YYVG HKGK FGHE FLEF EFRP DGKL RYAN NSNY KNDV MIRK EAYV HKSV MEEL KRII DDSE ITKE DDAL WPPP DRVG RQEL EIVI GDEH ISFT TSKI GSLI DVNQ SKDP EGLR VFYY LVQD LKCL VFSL IGLH FKIK PI
Gene ID:	4116
Uniprot:	P61326
Cellular Location:	Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:	12kDa/17kDa
Observed MW:	17kDa

Synonyms:	MAGOH, MAGOH1, MAGOHA
Background:	Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts.

UniProt Protein Function:	MAGOH: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Belongs to the mago nashi family.
UniProt Protein Details:	Protein type:RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 1p32.3 Cellular Component: nucleoplasm; nuclear speck; nucleus; cytosol Molecular Function:protein binding Biological Process: regulation of translation; transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of alternative nuclear mRNA splicing, via spliceosome
NCBI Summary:	Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]
UniProt Code:	P61326
NCBI GenInfo Identifier:	47117708
NCBI Gene ID:	4116
NCBI Accession:	P61326.1
UniProt Secondary Accession:	P61326,O35169, P50606, Q5SW69, B1ARP8, B2R5A2,
UniProt Related Accession:	P61326
Molecular Weight:	146
NCBI Full Name:	Protein mago nashi homolog
NCBI Synonym Full Names:	mago-nashi homolog, proliferation-associated (Drosophila)
NCBI Official Symbol:	MAGOH
NCBI Official Synonym Symbols:	MAGOH1; MAGOHA
NCBI Protein Information:	protein mago nashi homolog
UniProt Protein Name:	Protein mago nashi homolog
Protein Family:	Protein mago nashi
UniProt Gene Name:	MAGOH
UniProt Entry Name:	MGN_HUMAN

Western blot analysis of extracts of various cell lines, using MAGOH antibody (CAB6035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

MAGOH Antibody (PACO10348)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)