Anti-MALT1 Antibody (CAB2144)

Antibody Name:	Anti-MALT1 Antibody
Antibody SKU:	CAB2144
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 125-450 of human MALT1 (NP_776216.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 125-450 of human MALT1 (NP_776216.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGIK ITVN PESK AVLA GQFV KLCC RATG HPFV QYQW FKMN KEIP NGNT SELI FNAV HVKD AGFY VCRV NNNF TFEF SQWS QLDV CDIP ESFQ RSVD GVSE SKLQ ICVE PTSQ KLMP GSTL VLQC VAVG SPIP HYQW FKNE LPLT HETK KLYM VPYV DLEH QGTY WCHV YNDR DSQD SKKV EIII DELN NLGH PDNK EQTT DQPL AKDK VALL IGNM NYRE HPKL KAPL VDVY ELTN LLRQ LDFK VVSL LDLT EYEM RNAV DEFL LLLD KGVY GLLY YAGH GYEN FGNS FMVP VDAP NPYR SENC LCVQ NILK LMQE KETG LNVF LL
Gene ID:	10892
Uniprot:	Q9UDY8
Cellular Location:	Cytoplasm, Nucleus, perinuclear region
Calculated MW:	91kDa/92kDa
Observed MW:	102kDa

Synonyms:

MALT1, IMD12, MLT, MLT1, PCASP1

Background:

This gene has been found to be recurrently rearranged in chromosomal translocation with two other genes - baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) and immunoglobulin heavy chain locus - in mucosa-associated lymphoid tissue lymphomas. The protein encoded by this gene may play a role in NF-kappaB activation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	MALT1: Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. Binds through its Ig-like domains to BCL10. Forms oligomers which bind to TRAF6. Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung. Belongs to the peptidase C14B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Apoptosis; EC 3.4.22.-; Ligase; Nuclear export; Protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 18q21.32 Cellular Component: cytoplasm; cytosol; nucleolus; nucleus; plasma membrane; protein complex Molecular Function:kinase activator activity; peptidase activity; protein binding; protein self-association; signal transducer activity; ubiquitin-protein ligase activity Biological Process: activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; nuclear export; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 production; positive regulation of T cell activation; positive regulation of T cell cytokine production; protein oligomerization; proteolysis; regulation of apoptosis; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway Disease: Immunodeficiency 12
NCBI Summary:	This gene has been found to be recurrently rearranged in chromosomal translocation with two other genes - baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) and immunoglobulin heavy chain locus - in mucosa-associated lymphoid tissue lymphomas. The protein encoded by this gene may play a role in NF-kappaB activation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UDY8
NCBI GenInfo Identifier:	20455075
NCBI Gene ID:	10892
NCBI Accession:	Q9UDY8.1
UniProt Secondary Accession:	Q9UDY8,Q9NTB7, Q9ULX4,
UniProt Related Accession:	Q9UDY8
Molecular Weight:	91,081 Da
NCBI Full Name:	Mucosa-associated lymphoid tissue lymphoma translocation protein 1
NCBI Synonym Full Names:	MALT1 paracaspase
NCBI Official Symbol:	MALT1
NCBI Official Synonym Symbols:	MLT; MLT1; IMD12; PCASP1
NCBI Protein Information:	mucosa-associated lymphoid tissue lymphoma translocation protein 1
UniProt Protein Name:	Mucosa-associated lymphoid tissue lymphoma translocation protein 1
UniProt Synonym Protein Names:	MALT lymphoma-associated translocation; Paracaspase
Protein Family:	Mucosa-associated lymphoid tissue lymphoma translocation protein
UniProt Gene Name:	MALT1

	Western blot analysis of extracts of various cell lines, using MALT1 antibody (CAB2144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using MALT1 antibody (CAB2144) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using MALT1 antibody (CAB2144) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using MALT1 antibody (CAB2144) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

MALT1 Antibody (PACO02377)

MALT1 Antibody (PACO10352)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)