Anti-MAP1LC3C Antibody (CAB8295)

Antibody Name:	MAP1LC3C Rabbit Polyclonal Antibody
Antibody SKU:	CAB8295
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human MAP1LC3C (NP_001004343.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, OVCAR3, HepG2, 293T, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human MAP1LC3C (NP_001004343.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPPP QKIP SVRP FKQR KSLA IRQE EVAG IRAK FPNK IPVV VERY PRET FLPP LDKT KFLV PQEL TMTQ FLSI IRSR MVLR ATEA FYLL VNNK SLVS MSAT MAEI YRDY KDED GFVY MTYA SQET FGCL ESAA PRDG SSLE DRPC NPL
Gene ID:	440738
Uniprot:	Q9BXW4
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Calculated MW:	16kDa
Observed MW:	17kDa

Synonyms:	MAP1LC3C, ATG8J, LC3C
Background:

UniProt Protein Function:	LC3C: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes.
UniProt Protein Details:	Protein type:Vesicle; Autophagy; Microtubule-binding; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 1q43 Cellular Component: microtubule; extrinsic to membrane; endomembrane system; autophagic vacuole; cytoplasmic vesicle; cytosol Molecular Function:protein binding; microtubule binding; GABA receptor binding Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
NCBI Summary:	Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]
UniProt Code:	Q9BXW4
NCBI GenInfo Identifier:	84028113
NCBI Gene ID:	440738
NCBI Accession:	Q9BXW4.1
UniProt Secondary Accession:	Q9BXW4,A0PJY8, A2RUP0,
UniProt Related Accession:	Q9BXW4
Molecular Weight:	16,852 Da
NCBI Full Name:	Microtubule-associated proteins 1A/1B light chain 3C
NCBI Synonym Full Names:	microtubule-associated protein 1 light chain 3 gamma
NCBI Official Symbol:	MAP1LC3C
NCBI Official Synonym Symbols:	LC3C; ATG8J
NCBI Protein Information:	microtubule-associated proteins 1A/1B light chain 3C; LC3-like protein 2; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; MAP1A/MAP1B light chain 3 C; autophagy-related protein LC3 C; autophagy-related ubiquitin-like modifier LC3 C
UniProt Protein Name:	Microtubule-associated proteins 1A/1B light chain 3C
UniProt Synonym Protein Names:	Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma
Protein Family:	Microtubule-associated protein
UniProt Gene Name:	MAP1LC3C
UniProt Entry Name:	MLP3C_HUMAN

Anti-MAP1LC3C Polyclonal Antibody (CAB8295)

Western blot analysis of extracts of various cell lines, using MAP1LC3C antibody (CAB8295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)