Anti-MARCKSL1 Antibody (CAB7132)

Antibody Name:	Anti-MARCKSL1 Antibody
Antibody SKU:	CAB7132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MARCKSL1 (NP_075385.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, 22Rv1, HT-29, NCI-H460, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MARCKSL1 (NP_075385.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGSQ SSKA PRGD VTAE EAAG ASPA KANG QENG HVKS NGDL SPKG EGES PPVN GTDE AAGA TGDA IEPA PPSQ GAEA KGEV PPKE TPKK KKKF SFKK PFKL SGLS FKRN RKEG GGDS SASS PTEE EQEQ GEIG ACSD EGTA QEGK AAAT PESQ EPQA KGAE ASAA SEEE AGPQ ATEP STPS GPES GPTP ASAE QNE
Gene ID:	65108
Uniprot:	P49006
Cellular Location:	Cell membrane, Cytoplasm, Lipid-anchor, Membrane
Calculated MW:	19kDa
Observed MW:	35kDa

Synonyms:

MARCKSL1, F52, MACMARCKS, MLP, MLP1, MRP

Background:

This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10.

UniProt Protein Function:	MLP: Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems. Belongs to the MARCKS family.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 1p35.1 Cellular Component: cytoplasm; plasma membrane Molecular Function:calmodulin binding; actin binding Biological Process: positive regulation of cell proliferation
NCBI Summary:	This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10. [provided by RefSeq, Jun 2012]
UniProt Code:	P49006
NCBI GenInfo Identifier:	1346576
NCBI Gene ID:	65108
NCBI Accession:	P49006.2
UniProt Related Accession:	P49006
Molecular Weight:
NCBI Full Name:	MARCKS-related protein
NCBI Synonym Full Names:	MARCKS like 1
NCBI Official Symbol:	MARCKSL1
NCBI Official Synonym Symbols:	F52; MLP; MRP; MLP1; MACMARCKS
NCBI Protein Information:	MARCKS-related protein
UniProt Protein Name:	MARCKS-related protein
UniProt Synonym Protein Names:	MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate; Mac-MARCKS; MacMARCKS
Protein Family:	MIP-related peptides
UniProt Gene Name:	MARCKSL1
UniProt Entry Name:	MRP_HUMAN

	Western blot analysis of extracts of various cell lines, using MARCKSL1 antibody (CAB7132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using MARCKSL1 antibody (CAB7132). Blue: DAPI for nuclear staining.

Antibodies

MARCKSL1 Antibody (PACO10392)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)