Western blot analysis of various lysates, using MARCKSL1 Rabbit pAb (CAB24471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The MARCKSL1 Polyclonal Antibody (CAB24471) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the Marcksl1 protein, this antibody enables detection and analysis in various cell types, making it an essential tool for studies in cell biology and cancer research.Marcksl1, also known as Myristoylated alanine-rich C-kinase substrate-like 1, is a key regulator of cell motility and has been implicated in cancer progression and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MARCKSL1 Polyclonal Antibody
SKU:
CAB24471
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MARCKSL1, F52, MACMARCKS, MLP, MLP1, MRP, MARCKS like 1
Positive Sample:
293T, SH-SY5Y, Mouse thymus, Rat testis
Cellular Localization:
Cell Membrane, Cytoplasm, Lipid-Anchor, Membrane.
Calculated MW:
19kDa
Observed MW:
40kDa
This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10.
Purification Method
Affinity purification
Gene ID
65108
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using MARCKSL1 Rabbit pAb (CAB24471) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
