Anti-MATR3 Antibody (CAB5905)

Antibody Name:	Anti-MATR3 Antibody
Antibody SKU:	CAB5905
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 747-847 of human MATR3 (NP_001181884.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HepG2, MCF7, K-562, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 747-847 of human MATR3 (NP_001181884.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSEN ADDP NKDT SENA DGQS DENK DDYT IPDE YRIG PYQP NVPV GIDY VIPK TGFY CKLC SLFY TNEE VAKN THCS SLPH YQKL KKFL NKLA EERR QKKE T
Gene ID:	9782
Uniprot:	P43243
Cellular Location:	Nucleus matrix
Calculated MW:	62kDa/94kDa
Observed MW:	125kDa

Synonyms:

MATR3, ALS21, MPD2, VCPDM, matrin-3

Background:

This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. Mutations of this gene are associated with distal myopathy 2, which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants, including read-through transcripts composed of the upstream small nucleolar RNA host gene 4 (non-protein coding) and matrin 3 gene sequence, have been identified. Pseudogenes of this gene are located on chromosomes 1 and X.

UniProt Protein Function:	matrin 3: an acidic protein that is a component of the nuclear matrix. May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network.
UniProt Protein Details:	Protein type:RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 5q31.2 Cellular Component: membrane; nuclear inner membrane; nucleoplasm Molecular Function:protein binding; structural molecule activity Disease: Amyotrophic Lateral Sclerosis 21
NCBI Summary:	This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. Mutations of this gene are associated with distal myopathy 2, which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants, including read-through transcripts composed of the upstream small nucleolar RNA host gene 4 (non-protein coding) and matrin 3 gene sequence, have been identified. Pseudogenes of this gene are located on chromosomes 1 and X. [provided by RefSeq, Aug 2013]
UniProt Code:	P43243
NCBI GenInfo Identifier:	12643409
NCBI Gene ID:	9782
NCBI Accession:	P43243.2
UniProt Secondary Accession:	P43243,Q9UHW0, Q9UQ27, B7ZAV5, D3DQC3,
UniProt Related Accession:	P43243
Molecular Weight:	62,576 Da
NCBI Full Name:	Matrin-3
NCBI Synonym Full Names:	matrin 3
NCBI Official Symbol:	MATR3
NCBI Official Synonym Symbols:	MPD2; ALS21; VCPDM
NCBI Protein Information:	matrin-3
UniProt Protein Name:	Matrin-3
Protein Family:	Matrin
UniProt Gene Name:	MATR3
UniProt Entry Name:	MATR3_HUMAN

	Western blot analysis of extracts of various cell lines, using MATR3 antibody (CAB5905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat testis using MATR3 antibody (CAB5905) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using MATR3 antibody (CAB5905) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using MATR3 antibody (CAB5905) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using MATR3 antibody (CAB5905) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

MATR3 Antibody (PACO10402)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)