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Anti-SUMO2 Antibody (CAB2571)

SKU:
CAB2571
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 2 Anti-SUMO2 Antibody CAB2571
  • Signal Transduction Antibodies 2 Anti-SUMO2 Antibody CAB2571
€139 - €419
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Description

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Antibody Name:Anti-SUMO2 Antibody
Antibody SKU:CAB2571
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human SUMO2 (NP_008868.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:PC-12, 293T, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human SUMO2 (NP_008868.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADE KPKE GVKT ENND HINL KVAG QDGS VVQF KIKR HTPL SKLM KAYC ERQG LSMR QIRF RFDG QPIN ETDT PAQL EMED EDTI DVFQ QQTG GVY
Gene ID:6613
Uniprot:P61956
Cellular Location:Nucleus, PML body
Calculated MW:8kDa/10kDa
Observed MW:17kDa
Synonyms:SUMO2, HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
Background:This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UniProt Protein Function:SUMO2: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Homotrimer (Potential). Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25. Broadly expressed. Belongs to the ubiquitin family. SUMO subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; PML body; nucleus

Molecular Function:protein binding; ubiquitin protein ligase binding

Biological Process: protein sumoylation; cellular protein metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; post-translational protein modification

NCBI Summary:This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:P61956
NCBI GenInfo Identifier:378405233
NCBI Gene ID:6613
NCBI Accession:P61956.3
UniProt Secondary Accession:P61956,P55855, Q32Q42, Q6IPZ6, Q96HK1, B2R4I2,
UniProt Related Accession:P61956
Molecular Weight:95
NCBI Full Name:Small ubiquitin-related modifier 2
NCBI Synonym Full Names:small ubiquitin-like modifier 2
NCBI Official Symbol:SUMO2  
NCBI Official Synonym Symbols:HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2  
NCBI Protein Information:small ubiquitin-related modifier 2; sentrin 2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 2
UniProt Protein Name:Small ubiquitin-related modifier 2
UniProt Synonym Protein Names:HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3B
Protein Family:Small ubiquitin-related modifier
UniProt Gene Name:SUMO2  
UniProt Entry Name:SUMO2_HUMAN
Anti-SUMO2 Antibody (CAB2571)Western blot analysis of extracts of various cell lines, using SUMO2 antibody (CAB2571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-SUMO2 Antibody (CAB2571)Immunofluorescence analysis of U2OS cells using SUMO2 antibody (CAB2571). Blue: DAPI for nuclear staining.

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