Anti-MAVS Antibody (CAB5764)

Antibody Name:	Anti-MAVS Antibody
Antibody SKU:	CAB5764
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human MAVS (NP_065797.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, RAW264.7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human MAVS (NP_065797.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPFA EDKT YKYI CRNF SNFC NVDV VEIL PYLP CLTA RDQD RLRA TCTL SGNR DTLW HLFN TLQR R
Gene ID:	57506
Uniprot:	Q7Z434
Cellular Location:	Mitochondrion, Mitochondrion outer membrane, Peroxisome
Calculated MW:	14kDa/15kDa/17kDa/40kDa/56kDa
Observed MW:	70kDa

Synonyms:	CARDIF, IPS-1, IPS1, VISA, MAVS
Background:	This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MAVS: Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Present in T-cells, monocytes, epithelial cells and hepatocytes. Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Membrane protein, integral Chromosomal Location of Human Ortholog: 20p13 Cellular Component: mitochondrial membrane; mitochondrial outer membrane; mitochondrion; peroxisomal membrane Molecular Function:CARD domain binding; protein binding; protein kinase binding; signal transducer activity Biological Process: activation of innate immune response; defense response to bacterium; defense response to virus; innate immune response; negative regulation of interferon type I production; negative regulation of viral genome replication; positive regulation of defense response to virus by host; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-8 production; positive regulation of protein amino acid phosphorylation; positive regulation of protein import into nucleus, translocation; positive regulation of transcription factor activity; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; signal transduction
NCBI Summary:	This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:	Q7Z434
NCBI GenInfo Identifier:	47115748
NCBI Gene ID:	57506
NCBI Accession:	Q7Z434.2
UniProt Secondary Accession:	Q7Z434,Q2HWT5, Q3I0Y2, Q5T7I6, Q86VY7, Q9H1H3, Q9H4Y1 A8K6X0, B2BD33, B2BD34, F5H6C8, M1P2Z0,
UniProt Related Accession:	Q7Z434
Molecular Weight:	15,012 Da
NCBI Full Name:	Mitochondrial antiviral-signaling protein
NCBI Synonym Full Names:	mitochondrial antiviral signaling protein
NCBI Official Symbol:	MAVS
NCBI Official Synonym Symbols:	IPS1; VISA; IPS-1; CARDIF
NCBI Protein Information:	mitochondrial antiviral-signaling protein
UniProt Protein Name:	Mitochondrial antiviral-signaling protein
UniProt Synonym Protein Names:	CARD adapter inducing interferon beta; Cardif; Interferon beta promoter stimulator protein 1; IPS-1; Putative NF-kappa-B-activating protein 031N; Virus-induced-signaling adapter; VISA
Protein Family:	Mitochondrial antiviral-signaling protein
UniProt Gene Name:	MAVS
UniProt Entry Name:	MAVS_HUMAN

	Western blot analysis of extracts of various cell lines, using MAVS antibody (CAB5764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunofluorescence analysis of NIH-3T3 cells using MAVS Rabbit pAb (CAB5764) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

MAVS Antibody (PACO10403)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)