null

Anti-MDFI Antibody (CAB13709)

SKU:
CAB13709
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
  • Developmental Biology Anti-MDFI Antibody CAB13709
  • Developmental Biology Anti-MDFI Antibody CAB13709
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MDFI Antibody
Antibody SKU:CAB13709
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MYQV SGQR PSGC DAPY GAPS AAPG PAQT LSLL PGLE VVTG STHP AEAA PEEG SLEE AATP MPQG NGPG IPQG LDST DLDV PTEA VTCQ PQGN PLGC TPLL PNDS GHPS ELGG TRRA GNGA LGGP KAHR KLQT HPSL ASQG SKKS KSSS KSTT SQIP LQAQ
Gene ID:4188
Uniprot:Q99750
Cellular Location:Cytoplasm, Nucleus
Calculated MW:25kDa
Observed MW:35kDa
Synonyms:MDFI, I-MF, I-mfa
Background:This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.
UniProt Protein Function:Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.
NCBI Summary:This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]
UniProt Code:Q99750
NCBI GenInfo Identifier:663853311
NCBI Gene ID:4188
NCBI Accession:NP_001287733.1
UniProt Related Accession:Q99750
Molecular Weight:25 KD
NCBI Full Name:myoD family inhibitor isoform 1
NCBI Synonym Full Names:MyoD family inhibitor
NCBI Official Symbol:MDFI  
NCBI Official Synonym Symbols:I-MF; I-mfa  
NCBI Protein Information:myoD family inhibitor
UniProt Protein Name:MyoD family inhibitor
UniProt Synonym Protein Names:Myogenic repressor I-mf
Protein Family:MyoD family inhibitor
UniProt Gene Name:MDFI  
Anti-MDFI Antibody (CAB13709)Western blot analysis of extracts of various cell lines, using MDFI antibody (CAB13709) at 1:3000 dilution.
Anti-MDFI Antibody (CAB13709)Immunofluorescence analysis of U-2 OS cells using MDFI Rabbit pAb (CAB13709) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Related Products