Western blot analysis of extracts from wild type(WT) and ME1 knockdown (KD) MCF7(KD) cells, using ME1 antibody (CAB23068) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] ME1 Polyclonal Antibody (CAB23068) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been rigorously validated for use in Western blot applications with human samples. Its high specificity and sensitivity make it a reliable choice for detecting and quantifying ME1 in different cell types and tissues.ME1, also known as malic enzyme 1, is an enzyme involved in the citric acid cycle and is essential for the production of NADPH, a key molecule in cellular redox balance and biosynthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KD Validated] ME1 Polyclonal Antibody
SKU:
CAB23068
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MES, HUMNDME, [KD Validated] ME1
Positive Sample:
MCF7
Cellular Localization:
Cytoplasm.
Calculated MW:
64kDa
Observed MW:
64kDa
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Purification Method
Affinity purification
Gene ID
4199
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and ME1 knockdown (KD) MCF7 cells, using [KD Validated] ME1 Rabbit pAb (CAB23068) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
