Anti-ME1 Antibody (CAB3956)

Antibody Name:	Anti-ME1 Antibody
Antibody SKU:	CAB3956
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, MCF7, Mouse heart, Mouse brain, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPE APRR RHTH QRGY LLTR NPHL NKDL AFTL EERQ QLNI HGLL PPSF NSQE IQVL RVVK NFEH LNSD FDRY LLLM DLQD RNEK LFYR VLTS DIEK FMPI VYTP TVGL ACQQ YSLV FRKP RGLF ITIH DRGH IASV LNAW PEDV IKAI VVTD GERI LGLG DLGC NGMG IPVG KLAL YTAC GGMN PQEC LPVI LDVG TENE ELLK DPLY IGLR QRRV RGSE YDDF LDEF MEAV SSKY GMNC LIQF EDFA NVNA FRLL NKYR NQYC TFND DIQG TASV AVAG LLAA LRIT KNKL SDQT ILFQ
Gene ID:	4199
Uniprot:	P48163
Cellular Location:	Cytoplasm
Calculated MW:	55kDa/64kDa
Observed MW:	64kDa

Synonyms:

ME1, HUMNDME, MES

Background:

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

UniProt Protein Function:	ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.1.1.40; Carbohydrate Metabolism - pyruvate Chromosomal Location of Human Ortholog: 6q12 Cellular Component: mitochondrion; cytosol Molecular Function:malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; ADP binding; NADP binding; NAD binding; oxaloacetate decarboxylase activity Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization
NCBI Summary:	This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
UniProt Code:	P48163
NCBI GenInfo Identifier:	1346484
NCBI Gene ID:	4199
NCBI Accession:	P48163.1
UniProt Secondary Accession:	P48163,Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3 Q9UIY4, B4DZ70,
UniProt Related Accession:	P48163
Molecular Weight:	572
NCBI Full Name:	NADP-dependent malic enzyme
NCBI Synonym Full Names:	malic enzyme 1, NADP(+)-dependent, cytosolic
NCBI Official Symbol:	ME1
NCBI Official Synonym Symbols:	MES; HUMNDME
NCBI Protein Information:	NADP-dependent malic enzyme; NADP-ME; malate dehydrogenase; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; pyruvic-malic carboxylase
UniProt Protein Name:	NADP-dependent malic enzyme
UniProt Synonym Protein Names:	Malic enzyme 1
Protein Family:	NADP-dependent malic enzyme
UniProt Gene Name:	ME1
UniProt Entry Name:	MAOX_HUMAN

Western blot analysis of extracts of various cell lines, using ME1 antibody (CAB3956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

ME1 Antibody (PACO04312)

ME1 Antibody (PACO10433)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)