Description
MEF2A (Phospho-Thr312)Transcription Factor Activity Assay
The MEF2A Phospho Thr312 Transcription Factor Activity Assay is a highly sensitive and specific kit designed for detecting MEF2A phosphorylation at Thr312 in various samples such as cell lysates and tissue extracts. This assay provides accurate and reproducible results, making it suitable for a wide range of research applications.MEF2A is a key transcription factor that plays a crucial role in regulating gene expression involved in various cellular processes, including cell differentiation, proliferation, and apoptosis. Phosphorylation of MEF2A at Thr312 is essential for its transcriptional activity and plays a critical role in cell signaling pathways.
Understanding the activity of MEF2A at the molecular level is important for studying its role in different biological processes and its implications in various diseases such as cardiovascular disorders, muscular dystrophy, and neurodegenerative diseases. The MEF2A Phospho Thr312 Transcription Factor Activity Assay provides researchers with a valuable tool for investigating MEF2A function and its potential as a therapeutic target.
| Product Name: | MEF2A (Phospho-Thr312)Transcription Factor Activity Assay |
| Product Code: | TFAB00052 |
| Target: | MEF2A (Phospho-Thr312) |
| Synonyms: | Serum response factor-like protein 1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie MEF2A (Phospho-Thr312) transcription factor activity assay allows for the detection and qualitative analysis of MEF2A (Phospho-Thr312) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | MEF2A: a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. Five alternatively spliced isoforms have been reported. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 15q26 Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:chromatin binding; histone acetyltransferase binding; histone deacetylase binding; protein binding; protein heterodimerization activity; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; SMAD binding; transcription activator binding; transcription factor activity Biological Process: apoptosis; dendrite morphogenesis; heart development; innate immune response; MAPKKK cascade; mitochondrial genome maintenance; mitochondrion distribution; muscle cell differentiation; muscle development; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent; ventricular cardiac myofibril development Disease: Coronary Artery Disease, Autosomal Dominant, 1 |
| NCBI Summary: | The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010] |
| UniProt Code: | Q02078 |
| NCBI GenInfo Identifier: | 1170908 |
| NCBI Gene ID: | 4205 |
| NCBI Accession: | Q02078.1 |
| UniProt Secondary Accession: | Q02078,O43814, Q14223, Q14224, Q59GX4, Q7Z6C9, Q96D14 B4DFQ7, F6XG23, |
| UniProt Related Accession: | Q02078 |
| Molecular Weight: | 45,570 Da |
| NCBI Full Name: | Myocyte-specific enhancer factor 2A |
| NCBI Synonym Full Names: | myocyte enhancer factor 2A |
| NCBI Official Symbol: | MEF2A |
| NCBI Official Synonym Symbols: | mef2; ADCAD1; RSRFC4; RSRFC9 |
| NCBI Protein Information: | myocyte-specific enhancer factor 2A |
| UniProt Protein Name: | Myocyte-specific enhancer factor 2A |
| UniProt Synonym Protein Names: | Serum response factor-like protein 1 |
| Protein Family: | Myocyte-specific enhancer factor |
| UniProt Gene Name: | MEF2A |
| UniProt Entry Name: | MEF2A_HUMAN |
