The MEF2D Antibody (CAB16398) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting MEF2D in human samples, making it an optimal choice for Western blot applications.MEF2D is a key player in the regulation of gene expression and signal transduction pathways that influence cell differentiation, survival, and function. Its involvement in multiple physiological processes underscores its importance in developmental biology, neurobiology, and immunology research.
This antibody is validated for use in WB, ELISA, IHC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MEF2D Antibody
SKU:
CAB16398
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEF2D
Positive Sample:
HeLa, U-251MG, Mouse Brain
Cellular Localization:
Nucleus.
Calculated MW:
56kDa
Observed MW:
54kDa
This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4209
RRID
AB_2770335
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MEF2D Rabbit pAb (CAB16398) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using MEF2D Rabbit pAb (CAB16398) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
