The MEK1 Monoclonal Antibody (CAB19565) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting MEK1 in human samples, making it suitable for Western blot applications.MEK1 is a critical component of the MAPK/ERK pathway, which is often dysregulated in cancer, making it a promising target for therapeutic interventions. By targeting MEK1, researchers can gain valuable insights into the mechanisms underlying tumorigenesis and potentially develop novel treatment strategies for various types of cancer.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MEK1 Monoclonal Antibody
SKU:
CAB19565
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0044
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1
Positive Sample:
HeLa, HepG2, A-431, Mouse brain, Mouse lung, Rat lung
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method
Affinity purification
Gene ID
5604
RRID
AB_2862670
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MEK1 Rabbit mAb (CAB19565) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Immunofluorescence analysis of C6 cells using MEK1 Rabbit mAb (CAB19565). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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