Anti-Phospho-MEK1-S298 Antibody (CABP1020)

SKU:
CABP1020
€129 - €389

Description

Technical Manual

Anti-Phospho-MEK1-S298 Antibody

Product Name:Anti-Phospho-MEK1-S298 Antibody
Product SKU:CABP1020
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Immunogen:A phospho specific peptide corresponding to residues surrounding S298 of human MEK1
Sequence:

Email for sequence

Gene ID:5604
Swiss Prot:Q02750
Synonyms:CFC3; MAPKK1; MEK1; MKK1; PRKMK1
Calculated MW:45kDa
Observed MW:45KDa
Reactivity:Human, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting 
Positive Samples:HeLa, C6
Cellular Location:

Product Images

Anti-Phospho-MEK1-S298 Antibody (CABP1020)

Western blot - Phospho-MEK1-S298 Rabbit mAb (CABP1020)

Uniprot Information

UniProt Protein Function:MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
UniProt Protein Details:

Protein type:EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q22.1-q22.33

Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; axon; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function:MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; protein heterooligomerization; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; stress-activated MAPK cascade; cell motility involved in cell locomotion; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; chemotaxis; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; cell proliferation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; response to oxidative stress; positive regulation of Ras protein signal transduction; cell motility; positive regulation of cell differentiation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration

Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3

NCBI Summary:The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
UniProt Code:Q02750
NCBI GenInfo Identifier:400274
NCBI Gene ID:5604
NCBI Accession:Q02750.2
UniProt Related Accession:Q02750
Molecular Weight:393
NCBI Full Name:Dual specificity mitogen-activated protein kinase kinase 1
NCBI Synonym Full Names:mitogen-activated protein kinase kinase 1
NCBI Official Symbol:MAP2K1 
NCBI Official Synonym Symbols:CFC3; MEK1; MKK1; MAPKK1; PRKMK1 
NCBI Protein Information:dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
UniProt Protein Name:Dual specificity mitogen-activated protein kinase kinase 1
UniProt Synonym Protein Names:ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1
UniProt Gene Name:MAP2K1 
UniProt Entry Name:MP2K1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
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