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Anti-MSLN Antibody (CAB6183)

SKU:
CAB6183
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 10 Anti-MSLN Antibody CAB6183
  • Cell Biology Antibodies 10 Anti-MSLN Antibody CAB6183
€139 - €419
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Description

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Antibody Name:Anti-MSLN Antibody
Antibody SKU:CAB6183
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-296 of human MSLN (NP_001170826.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human
Positive Samples:HepG2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-296 of human MSLN (NP_001170826.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAGE TGQE AAPL DGVL ANPP NISS LSPR QLLG FPCA EVSG LSTE RVRE LAVA LAQK NVKL STEQ LRCL AHRL SEPP EDLD ALPL DLLL FLNP DAFS GPQA CTRF FSRI TKAN VDLL PRGA PERQ RLLP AALA CWGV RGSL LSEA DVRA LGGL ACDL PGRF VAES AEVL LPRL VSCP GPLD QDQQ EAAR AALQ GGGP PYGP PSTW SVST MDAL RGLL PVLG QPII RSIP QGIV AAWR QRSS RDPS WRQP ERTI LRPR FRRE
Gene ID:10232
Uniprot:Q13421
Cellular Location:Cell membrane, GPI-anchor, Golgi apparatus, Lipid-anchor, Secreted
Calculated MW:67kDa/68kDa/71kDa
Observed MW:69kDa
Synonyms:MSLN, MPF, SMRP
Background:This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
UniProt Protein Function:MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane

Molecular Function:protein binding

Biological Process: pancreas development; cell adhesion

NCBI Summary:This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
UniProt Code:Q13421
NCBI GenInfo Identifier:116242654
NCBI Gene ID:10232
NCBI Accession:Q13421.2
UniProt Secondary Accession:Q13421,Q14859, Q4VQD5, Q96GR6, Q96KJ5, Q9BR17, Q9BTR2 Q9UCB2, Q9UK57, D3DU65,
UniProt Related Accession:Q13421
Molecular Weight:67,940 Da
NCBI Full Name:Mesothelin
NCBI Synonym Full Names:mesothelin
NCBI Official Symbol:MSLN  
NCBI Official Synonym Symbols:MPF; SMRP  
NCBI Protein Information:mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
UniProt Protein Name:Mesothelin
UniProt Synonym Protein Names:CAK1 antigen; Pre-pro-megakaryocyte-potentiating factorCleaved into the following 2 chains:Megakaryocyte-potentiating factor; MPF; Mesothelin, cleaved form
Protein Family:Mesothelin
UniProt Gene Name:MSLN  
UniProt Entry Name:MSLN_HUMAN
Anti-MSLN Antibody (CAB6183)Western blot analysis of extracts of various cell lines, using MSLN antibody (CAB6183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-MSLN Antibody (CAB6183)Immunofluorescence analysis of U2OS cells using MSLN antibody (CAB6183). Blue: DAPI for nuclear staining.

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