Anti-MEST Antibody (CAB6931)

Antibody Name:	Anti-MEST Antibody
Antibody SKU:	CAB6931
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST (NP_002393.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, BT-474, HT-29, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST (NP_002393.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HDYG DIVA QELL YRYK QNRS GRLT IKSL CLSN GGIF PETH RPLL LQKL LKDG GVLS PILT RLMN FFVF SRGL TPVF GPYT RPSE SELW DMWA GIRN NDGN LVID SLLQ YINQ RKKF RRRW VGAL ASVT IPIH FIYG PLDP VNPY PEFL ELYR KTLP RSTV SILD DHIS HYPQ LEDP MGFL NAYM GFIN SF
Gene ID:	4232
Uniprot:	Q5EB52
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	33kDa/37kDa/38kDa
Observed MW:	39kDa

Synonyms:

MEST, PEG1

Background:

This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.

UniProt Protein Function:	MEST: a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; EC 3.-.-.- Chromosomal Location of Human Ortholog: 7q32 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane Molecular Function:hydrolase activity Biological Process: response to retinoic acid; metabolic process; mesoderm development
UniProt Code:	Q5EB52
NCBI GenInfo Identifier:	145566794
NCBI Gene ID:
NCBI Accession:	Q5EB52.2
Molecular Weight:	~ 39kDa
NCBI Full Name:	Mesoderm-specific transcript homolog protein
UniProt Protein Name:	Mesoderm-specific transcript homolog protein
UniProt Synonym Protein Names:	Paternally-expressed gene 1 protein
Protein Family:	Mesoderm-specific transcript protein
UniProt Gene Name:	MEST
UniProt Entry Name:	MEST_HUMAN

	Western blot analysis of extracts of various cell lines, using MEST antibody (CAB6931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using MEST antibody (CAB6931). Blue: DAPI for nuclear staining.

Antibodies

MEST Antibody (PACO10458)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)