The Mouse gp130 ELISA (Small Sample Volume) is designed for the quantitative detection of Mouse gp130 levels in various biological samples. Glycoprotein 130 (gp130/IL6ST) is the shared signal-transducing receptor subunit for the IL-6 cytokine family, including IL-6, IL-11, IL-27, oncostatin M (OSM), leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), and cardiotrophin-1. Upon cytokine binding, gp130 homodimerizes or heterodimerizes with ligand-specific receptor subunits and activates JAK/STAT3, MAPK, and PI3K/Akt downstream signaling cascades that regulate hematopoiesis, acute-phase responses, cardiac protection, neural differentiation, and cell survival. Dysregulated gp130 signaling contributes to inflammatory diseases, cardiac hypertrophy, and several cancers including gastric carcinoma. Assay Genie's Mouse gp130 ELISA (Small Sample Volume) offers exceptional sensitivity and specificity with a sensitivity of 1.88 ng/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Mouse gp130 ELISA (Small Sample Volume) for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Mouse gp130 ELISA (Small Sample Volume)
SKU:
AEES03429
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
