The Mouse gp130 (Glucoprotein 130) ELISA Kit is a specialized assay designed to quantitatively measure the levels of gp130 in various biological samples. gp130, a crucial signaling receptor within the interleukin-6 (IL-6) family, plays a pivotal role in mediating diverse cellular processes, including immune responses, inflammation, and cell growth regulation. Through its involvement in intracellular signal transduction pathways, gp130 contributes to the modulation of critical biological functions and the maintenance of physiological homeostasis. Accurate quantification of gp130 levels is essential for unraveling its complex role in cellular signaling cascades, immune responses, and disease pathways. Understanding the precise expression and regulation of gp130 can provide valuable insights into the mechanisms underlying inflammatory conditions, autoimmune disorders, and various pathological states where aberrant signaling occurs. The Mouse gp130 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured with rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an excellent tool for researchers seeking to explore the functions of gp130 in biological systems.
Product Name:
Mouse gp130 (Glucoprotein 130) ELISA Kit
SKU:
AEES00261
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
