The PIWIL1 Antibody (CAB2150) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through rabbit immunization, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to PIWI-like Protein 1, it enables precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and RNA regulation studies.PIWI-like Protein 1, also known as PIWIL1, is essential for the biogenesis and function of PIWI-interacting RNAs (piRNAs), which play crucial roles in transposon silencing, germ cell development, and epigenetic regulation.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PIWIL1 Antibody
SKU:
CAB2150
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MIWI, PIWIL1
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
99kDa
Observed MW:
90kDa
Enables several functions, including RNA binding activity; mRNA cap binding complex binding activity; and polysome binding activity. Involved in negative regulation of transposition; piRNA metabolic process; and spermatogenesis, exchange of chromosomal proteins. Located in chromatoid body; dense body; and nucleus. Is expressed in genitourinary system. Orthologous to human PIWIL1 (piwi like RNA-mediated gene silencing 1).
Purification Method
Affinity purification
Gene ID
57749
RRID
AB_2764169
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PIWIL1 Rabbit pAb (CAB2150) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using PIWIL1 Rabbit pAb (CAB2150) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded Mouse testis using PIWIL1 Rabbit pAb (CAB2150) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded Rat testis using PIWIL1 Rabbit pAb (CAB2150) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
