Anti-MMP25 Antibody (CAB15886)

Antibody Name:	Anti-MMP25 Antibody
Antibody SKU:	CAB15886
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-540 of human MMP25 (NP_071913.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HT-29, Mouse lung, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-540 of human MMP25 (NP_071913.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SPRP ARLH RFWE GLPA QVRV VQAA YARH RDGR ILLF SGPQ FWVF QDRQ LEGG ARPL TELG LPPG EEVD AVFS WPQN GKTY LVRG RQYW RYDE AAAR PDPG YPRD LSLW EGAP PSPD DVTV SNAG DTYF FKGA HYWR FPKN SIKT EPDA PQPM GPNW LDCP APSS GPRA PRPP KATP VSET CDCQ CELN QAA
Gene ID:	64386
Uniprot:	Q9NPA2
Cellular Location:	Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular matrix, extracellular space
Calculated MW:	62kDa
Observed MW:	75kDa

Synonyms:

MMP25, MMP-25, MMP20, MMP20A, MMPL1, MT-MMP 6, MT-MMP6, MT6-MMP, MT6MMP, MTMMP6

Background:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.

UniProt Protein Function:	MMP25: May activate progelatinase A. Belongs to the peptidase M10A family.
UniProt Protein Details:	Protein type:Membrane protein, integral; EC 3.4.24.-; Membrane protein, GPI anchor; Cell surface; Activator; Protease Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: proteinaceous extracellular matrix; membrane; plasma membrane; integral to membrane Molecular Function:zinc ion binding; metalloendopeptidase activity; calcium ion binding Biological Process: hard palate development; proteolysis; inflammatory response
NCBI Summary:	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NPA2
NCBI GenInfo Identifier:	12585274
NCBI Gene ID:	64386
NCBI Accession:	Q9NPA2.1
UniProt Secondary Accession:	Q9NPA2,Q9H3Q0, D3DUA8,
UniProt Related Accession:	Q9NPA2
Molecular Weight:
NCBI Full Name:	Matrix metalloproteinase-25
NCBI Synonym Full Names:	matrix metallopeptidase 25
NCBI Official Symbol:	MMP25
NCBI Official Synonym Symbols:	MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
NCBI Protein Information:	matrix metalloproteinase-25; leukolysin; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metallopeptidase-like 1; matrix metalloproteinase-like 1; membrane-type 6 matrix metalloproteinase; membrane-type matrix metalloproteinase 6; membrane-type-6 matrix metalloproteinase
UniProt Protein Name:	Matrix metalloproteinase-25
UniProt Synonym Protein Names:	Leukolysin; Membrane-type matrix metalloproteinase 6; MT-MMP 6; MTMMP6; Membrane-type-6 matrix metalloproteinase; MT6-MMP; MT6MMP
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP25
UniProt Entry Name:	MMP25_HUMAN

	Western blot analysis of extracts of various cell lines, using MMP25 antibody (CAB15886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat spleen using MMP25 antibody (CAB15886) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using MMP25 antibody (CAB15886) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using MMP25 antibody (CAB15886) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using MMP25 antibody (CAB15886) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-MMP25 Antibody (CAB3032)	Human MMP-25 (Matrix Metalloproteinase 25) CLIA Kit (HUES00118)
	Human MMP-25 (Matrix Metalloproteinase 25) ELISA Kit (HUES01408)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)