Anti-MMP9 Antibody (CAB2095)

Antibody Name:	Anti-MMP9 Antibody [KO Validated]
Antibody SKU:	CAB2095
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	B-cell, MCF7, K-562, SW480, mouse liver, mouse lung, mouse thymus, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KYWR FSEG RGSR PQGP FLIA DKWP ALPR KLDS VFEE RLSK KLFF FSGR QVWV YTGA SVLG PRRL DKLG LGAD VAQV TGAL RSGR GKML LFSG RRLW RFDV KAQM VDPR SASE VDRM FPGV PLDT HDVF QYRE KAYF CQDR FYWR VSSR SELN QVDQ VGYV TYDI LQCP ED
Gene ID:	4318
Uniprot:	P14780
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	78kDa
Observed MW:	110kDa

Synonyms:

MMP9, CLG4B, GELB, MANDP2, MMP-9

Background:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

UniProt Protein Function:	MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-\|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.
UniProt Protein Details:	Protein type:Protease; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.35 Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region Molecular Function:collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity Biological Process: positive regulation of keratinocyte migration; extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; leukocyte migration; skeletal development; embryo implantation Disease: Metaphyseal Anadysplasia 2
NCBI Summary:	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
UniProt Code:	P14780
NCBI GenInfo Identifier:	269849668
NCBI Gene ID:	4318
NCBI Accession:	P14780.3
UniProt Secondary Accession:	P14780,Q3LR70, Q8N725, Q9H4Z1, Q9UCJ9, Q9UCL1, Q9UDK2 B2R7V9,
UniProt Related Accession:	P14780
Molecular Weight:	78,458 Da
NCBI Full Name:	Matrix metalloproteinase-9
NCBI Synonym Full Names:	matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
NCBI Official Symbol:	MMP9
NCBI Official Synonym Symbols:	GELB; CLG4B; MMP-9; MANDP2
NCBI Protein Information:	matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
UniProt Protein Name:	Matrix metalloproteinase-9
UniProt Synonym Protein Names:	92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP9
UniProt Entry Name:	MMP9_HUMAN

	Western blot analysis of extracts of various cell lines, using MMP9 antibody (CAB2095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody (CAB2095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of rat lung using MMP9 antibody (CAB2095) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-MMP9 Antibody (CAB0289)	Human MMP-9 (Matrix Metalloproteinase 9) CLIA Kit (HUES00828)
Anti-MMP9 Antibody (CAB11147)	Human Matrix metalloproteinase-9 (MMP9) ELISA Kit
Anti-MMP9 Antibody (CAB11391)	MMP-9 Colorimetric Cell-Based ELISA Kit
Anti-MMP9 Antibody (CAB11521)
MMP9 Antibody (PACO02045)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)