Description
mNeonGreen Polyclonal Antibody (CAB24858)
The mNeonGreen Polyclonal Antibody (CAB24858) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and sensitivity towards MNeonGreen, making it ideal for various applications such as immunofluorescence and flow cytometry.MNeonGreen is a stable and bright green fluorescent protein, making it an excellent choice for visualizing protein localization, tracking cell movements, and monitoring gene expression. The MNeonGreen Polyclonal Antibody enables researchers to efficiently detect and analyze MNeonGreen-tagged proteins in a variety of experimental settings, facilitating the study of cellular dynamics and protein interactions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Species independent samples.
| Product Name: | mNeonGreen Polyclonal Antibody |
| SKU: | CAB24858 |
| Size: | 20μL, 100μL |
| Reactivity: | Species independent |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MGRD LYDD DDKD PLEM VSKG EEDN MASL PATH ELHI FGSI NGVD FDMV GQGT GNPN DGYE ELNL KSTK GDLQ FSPW ILVP HIGY GFHQ YLPY PDGM SPFQ AAMV DGSG YQVH RTMQ FEDG ASLT VNYR YTYE GSHI KGEA QVKG TGFP ADGP VMTN SLTA ADWC RSKK TYPN DKTI ISTF KWSY TTGN GKRY RSTA RTTY TFAK PMAA NYLK NQPM YVFR KTEL KHS | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Positive Sample: | 293T |
| Calculated MW: | 30kDa |
| Observed MW: | 30kDa |
Mutations were performed on LanYFP, and mNeonGreen (GenBank login number KC295282) was identified. In addition to the additional EGFP type terminal 9, a total of 21 mutations were found relative to the tetramer LanYFP (F15I, R25Q, A45D, Q56H, F67Y, K79V, S100V, F115A, I118K, V140R, T141S, M143K, L144T, D156K, T158S, S163N, Q168R, V171A, N174T, I185Y, F192Y). It is a yellow green fluorescent protein derived from amphioxus. As a fusion protein, mNeonGreen is a very useful tool for reporting gene expression, tracking cell lines, and determining subcellular protein localization.On the protein sequence level, only 20-25% of the mNeonGreen sequences are consistent with ordinary GFP derivative sequences; MNeonGreen has a maximum excitation value at 506 nm and a maximum emission value at 517 nm (Shaner et al., 2013). MNeonGreen is compatible with most GFP filter sets. Obviously, when using a GFP filter, it is three times brighter than GFP; The optimization of filters can further increase their brightness,In addition, mNeonGreen appears to be more stable than EGFP and less sensitive to laser induced bleaching. Therefore, mNeonGreen is particularly suitable for confocal microscopy and super-resolution microscopy, especially when studying low-level expression of fusion proteins.
| Purification Method | Affinity purification |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from wild type (WT) and 293T cells transfected with mNeonGreen using mNeonGreen Rabbit pAb (CAB24858) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s. |
 | Immunofluorescence analysis of 293F-6Xhis-mNeonGreen-S-tag(C) and 293F cells using mNeonGreen Rabbit pAb(CAB24858) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
