Monkey EPO-IgM (Erythropoietin Immunoglobulin M) ELISA Kit
The Monkey EPO-IgM (Erythropoietin Immunoglobulin M) ELISA Kit from AssayGenie is a cutting-edge tool for precise measurement of EPO-IgM levels in monkey serum, plasma, cell culture supernatant and other biological samples. The kit delivers exceptional sensitivity and specificity, ensuring accurate quantification of EPO-IgM across a wide dynamic range and minimizing cross-reactivity with other immunoglobulin classes. EPO-IgM represents the primary antibody response to erythropoietin, a critical hematopoietic cytokine that regulates red blood cell production through JAK2-STAT5 signaling in bone marrow erythroid progenitors. Elevated EPO-IgM titers are clinically relevant in autoimmune conditions, certain hematologic disorders, and vaccine or therapeutic immunogenicity assessments in nonhuman primate models.
Equipped with this ELISA kit, researchers can delve deep into the intricate mechanisms governed by EPO-IgM and gain valuable insights into its involvement in different biological pathways. Unleash the potential of your monkey research with the AssayGenie Monkey EPO-IgM (Erythropoietin Immunoglobulin M) ELISA Kit empowering innovation and discovery in the realm of molecular biology.
Product Name:
Monkey EPO-IgM (Erythropoietin Immunoglobulin M) ELISA Kit
SKU:
AEFI04119
Reactivity:
Monkey
Detection Method:
Capture ELISA
Sample Type:
Serum, Plasma, Cell Culture Supernatant and Other Biological Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n=5)
87-100%
86-102%
85-101%
EDTA Plasma (n=5)
82-101%
86-101%
84-99%
Heparin Plasma (n=5)
82-94%
81-99%
81-88%
Recovery:
Matrix
Recovery Range (%)
Average (%)
Serum (n=5)
97-104
99
EDTA Plasma (n=5)
91-105
98
Heparin Plasma (n=5)
91-102
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate to room temperature for at least 30 minutes. Designate standard, sample and control wells on the pre-coated plate. Wash the plate twice before adding standards, samples, and controls.
2
Standard, Sample & Control Loading: Aliquot standard solutions, samples and Sample Dilution Buffer into the appropriate wells.
3
First Incubation & Wash: Seal the plate and incubate at 37°C for 90 minutes. Wash 2 times with Wash Buffer.
4
Biotin-labelled Antibody, SABC & Wash: Add Biotin-labelled antibody working solution and incubate at 37°C for 60 minutes. Wash 3 times. Add SABC working solution and incubate at 37°C for 30 minutes. Wash 5 times, allowing buffer to stay 1-2 minutes per wash.
5
Color Development: Add TMB substrate and incubate at 37°C in the dark for 10-20 minutes.
6
Stop Reaction & Reading: Add Stop Solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow whole blood to coagulate at room temperature (2 h) or 2-8°C overnight. Centrifuge at 1000 × g for 20 min and collect the supernatant. Store or use immediately.
Plasma
Collect in anticoagulant tubes (EDTA, citrate, or heparin), mix gently, and centrifuge within 30 min at 1000 × g, 2-8°C for 15 min. Store or assay as needed.
Tissue Homogenate
Homogenize tissue in 50 mM Tris + 0.9% NaCl + 0.1% SDS (pH 7.3) with protease inhibitors (e.g. 1 mM PMSF). Avoid NP-40, Triton X-100 and DTT. Disrupt by ultrasonic homogenization or freeze-thaw cycles, centrifuge at 5000 × g for 5 min and collect the supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes at 2-8°C and collect the clarified cell culture supernatant.
Cell Lysate
Lyse cells in lysis buffer with protease inhibitor (e.g. 1 mM PMSF) on ice for 30-60 minutes, or by ultrasonic treatment. Centrifuge at 10,000 rpm for 10 min at 2-8°C and collect the supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
