The MonoMethyl-Histone H3-K9 Antibody (CAB2358) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for detecting monomethylated Histone H3 at lysine 9 (H3K9me1) in human, mouse, and rat samples. It has been validated for use in techniques such as Western blot and immunofluorescence, allowing for accurate and reliable detection of this specific histone modification.Histone H3 lysine 9 methylation is a key epigenetic mark involved in regulating gene expression, chromatin structure, and cellular differentiation. Dysregulation of H3K9 methylation has been implicated in various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ChIP, ChIP-seq, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H3-K9 Antibody
SKU:
CAB2358
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ARKS T
Tested Applications:
WBIHC-PIF/ICCChIPChIP-seqELISADB
Recommended Dilution:
WB
1:500 - 1:1000
DB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2721265
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K9 Rabbit pAb (CAB2358) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (CAB2358).
Immunohistochemistry analysis of paraffin-embedded Mouse lung using MonoMethyl-Histone H3-K9 Rabbit pAb (CAB2358) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 Rabbit pAb (CAB2358). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 Rabbit pAb (CAB2358) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 Rabbit pAb (CAB2358) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K9 antibody (CAB2358) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
