Anti-MonoMethyl-Histone H4-K20 Antibody (CAB2370)

Antibody Name:	Anti-MonoMethyl-Histone H4-K20 Antibody
Antibody SKU:	CAB2370
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP ChIPseq
Reactivity:	Human, Mouse, Rat, Other (Wide Range)
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human MonoMethyl-Histone H4-K20

Application:	WB IHC IF IP ChIP ChIPseq
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:100
Reactivity:	Human, Mouse, Rat, Other (Wide Range)
Positive Samples:	HeLa, C2C12, C6

Immunogen:	A synthetic peptide of human MonoMethyl-Histone H4-K20
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	8370
Uniprot:	P62805
Cellular Location:	Chromosome, Nucleus
Calculated MW:	11kDa
Observed MW:	11kDa

Synonyms:

FO108, H4, H4/n, H4F2, H4FN, HIST2H4, Histone H4, HIST1H4A, HIST2H4A

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

UniProt Protein Function:	Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:	Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular location: Nucleus. Chromosome. Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35 Sequence similarities: Belongs to the histone H4 family. Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008]
UniProt Code:	P62805
NCBI GenInfo Identifier:	77539758
NCBI Gene ID:	554313
NCBI Accession:	NP_001029249.1
UniProt Secondary Accession:	P62805,P02304, P02305, Q6DRA9, Q6FGB8, Q6NWP7, A2VCL0
UniProt Related Accession:	P62805
Molecular Weight:	11,367 Da
NCBI Full Name:	histone H4
NCBI Synonym Full Names:	histone cluster 2, H4b
NCBI Official Symbol:	HIST2H4B
NCBI Official Synonym Symbols:	H4/o
NCBI Protein Information:	histone H4; histone 2, H4b
UniProt Protein Name:	Histone H4
Protein Family:	Histone
UniProt Gene Name:	HIST1H4A
UniProt Entry Name:	H4_HUMAN

	Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-K20 antibody (CAB2370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of C2C12 cells, using MonoMethyl-Histone H4-K20 antibody (CAB2370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H4-K20 antibody (CAB2370).
	Immunohistochemistry of paraffin-embedded human colon using MonoMethyl-Histone H4-K20 antibody (CAB2370) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H4-K20 antibody (CAB2370) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-Histone H4 Antibody (CAB1131)	Human Histone H4 (HIST1H4A) ELISA Kit
Anti-Acetyl-Histone H4-K12 Antibody (CAB14227)	Histone H4 Colorimetric Cell-Based ELISA Kit
Anti-Acetyl-Histone H4-K5 Antibody (CAB15233)
Anti-MonoMethyl-Histone H4-K5 Antibody (CAB17859)
Anti-DiMethyl-Histone H4-K20 Antibody (CAB2371)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)