Mouse 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) ELISA Kit
The Mouse 17-beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17B10) ELISA Kit is specifically designed for the accurate and sensitive detection of HSD17B10 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, providing reliable and reproducible results for various research applications.HSD17B10 is an important enzyme involved in the metabolism of steroid hormones, playing a crucial role in hormone regulation and various metabolic processes. Dysregulation of HSD17B10 has been linked to a range of diseases, including metabolic disorders, neurodegenerative diseases, and certain cancers, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its high-performance capabilities, the Mouse HSD17B10 ELISA Kit is a valuable tool for researchers looking to investigate the function and significance of HSD17B10 in health and disease. Its ease of use and accurate results make it a reliable choice for studying metabolic pathways, hormone regulation, and potential therapeutic targets in mouse models.
Product Name:
Mouse 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
