The Mouse ADP-ACRP30 (Adiponectin) CLIA Kit is a cutting-edge assay designed for the precise measurement of adiponectin levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Adiponectin, also known as ADP or ACRP30, is a key protein involved in regulating glucose levels and fatty acid breakdown in the body. Its levels have been linked to obesity, insulin resistance, and metabolic syndrome, making it a valuable biomarker for studying these conditions and developing potential treatment strategies.
Whether investigating metabolic disorders, obesity-related diseases, or cardiovascular conditions, the Mouse ADP-ACRP30 CLIA Kit provides researchers with a reliable tool for quantifying adiponectin levels and gaining valuable insights into physiological and pathological processes.
Product Name:
Mouse ADP/Acrp30 (Adiponectin) CLIA Kit
SKU:
MOES00002
Target:
Mouse ADP/Acrp30 (Adiponectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP/Acrp30. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ADP/Acrp30. You can calculate the concentration of Mouse ADP/Acrp30 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
89-104
94-111
Average (%)
96
97
101
1:4
Range (%)
95-111
88-103
88-101
Average (%)
101
95
94
1:8
Range (%)
84-98
99-116
91-103
Average (%)
91
106
96
1:16
Range (%)
102-116
87-99
103-117
Average (%)
109
93
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
0.98
5.0
0.51
1.06
4.84
Standard deviation
0.04
0.08
0.57
0.06
0.12
0.48
C V (%)
8.33
8.16
11.4
11.76
11.32
9.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ADP/Acrp30 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ADP/Acrp30 in samples. No significant cross-reactivity or interference between Mouse ADP/Acrp30 and analogues was observed.
