The Mouse ADP (Adiponectin) ELISA Kit is specifically designed for the quantitative detection of mouse adiponectin levels in serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Adiponectin is a key adipokine that plays a crucial role in regulating glucose and lipid metabolism, as well as inflammation and insulin sensitivity. It is an important biomarker for metabolic diseases such as obesity, type 2 diabetes, and cardiovascular disease, making it essential for studying these conditions and potential therapeutic interventions.Overall, the Mouse ADP (Adiponectin) ELISA Kit is a valuable tool for researchers looking to explore the role of adiponectin in various physiological and pathological processes in mouse models.
Product Name:
Mouse ADP/Acrp30 (Adiponectin) ELISA Kit
SKU:
MOES00623
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
