The Mouse ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit is a specialized assay designed for the quantitative detection of Alkaline Phosphatase (ALP) levels in biological samples obtained from mice. Alkaline Phosphatase is a crucial enzyme found in various tissues, including the liver, bone, and kidney, and plays a key role in several physiological processes, such as bone mineralization, phosphate metabolism, and liver function. Accurate measurement of ALP levels is essential for studying bone health, liver diseases, and kidney function in mouse models. The Mouse ALPL ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance, delivering accurate quantification of ALP levels in mouse samples. Trust Assay Genie's Mouse ALPL ELISA Kit for precise and dependable analysis of Alkaline Phosphatase in your research studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
